Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- ELISA [1]
- Other assay [2]
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- Product number
- HAH 002-01-02 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Butyrylcholinesterase Monoclonal Antibody (3E8)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- HAH 002-01-02 has specificity for butyrylcholinesterase from human serum or plasma. Crossreactivity to BtChE from other species has not been tested. HAH 002-01-02 has been successfully used in ELISA techniques. HAH 002-01-02 reacts with BtChE from normal serum when tested in sandwich ELISA. This antibody can be used as both the capture and detection antibody or an alternative antibody against BtChE can be used as capture antibody. In addition, it can be used in crossed immunoelectrophoresis followed by incubation of the gel with HAH 002-01-02. In immunoblotting after SDS-PAGE no reaction is seen with either the reduced or with the non-reduced form of BtChE. No reaction is seen with acetylcholinesterase from human nervous tissue and erythrocytes. The HAH 002-01-02 immunogen is Butyrylcholinesterase found in human plasma cells.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3E8
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, store in dark
Submitted references Comparison of Efficiency of Purification (from Human Plasma) of a Nerve Agent Adduct of Butyrylcholinesterase Between the Affinity Gel Method and Immunomagnetic Separation.
Simultaneous Time-concentration Analysis of Soman and VX Adducts to Butyrylcholinesterase and Albumin by LC-MS-MS.
Monoclonal antibodies to human butyrylcholinesterase reactive with butyrylcholinesterase in animal plasma.
Comparison of 5 monoclonal antibodies for immunopurification of human butyrylcholinesterase on Dynabeads: KD values, binding pairs, and amino acid sequences.
Detection of cresyl phosphate-modified butyrylcholinesterase in human plasma for chemical exposure associated with aerotoxic syndrome.
A high-throughput diagnostic method for measuring human exposure to organophosphorus nerve agents.
Immunomagnetic separation and quantification of butyrylcholinesterase nerve agent adducts in human serum.
Lee JY
Journal of chromatographic science 2018 Mar 1;56(3):248-253
Journal of chromatographic science 2018 Mar 1;56(3):248-253
Simultaneous Time-concentration Analysis of Soman and VX Adducts to Butyrylcholinesterase and Albumin by LC-MS-MS.
Lee JY, Kim C, Lee YH
Journal of analytical toxicology 2018 Jun 1;42(5):293-299
Journal of analytical toxicology 2018 Jun 1;42(5):293-299
Monoclonal antibodies to human butyrylcholinesterase reactive with butyrylcholinesterase in animal plasma.
Peng H, Brimijoin S, Hrabovska A, Krejci E, Blake TA, Johnson RC, Masson P, Lockridge O
Chemico-biological interactions 2016 Jan 5;243:82-90
Chemico-biological interactions 2016 Jan 5;243:82-90
Comparison of 5 monoclonal antibodies for immunopurification of human butyrylcholinesterase on Dynabeads: KD values, binding pairs, and amino acid sequences.
Peng H, Brimijoin S, Hrabovska A, Targosova K, Krejci E, Blake TA, Johnson RC, Masson P, Lockridge O
Chemico-biological interactions 2015 Oct 5;240:336-45
Chemico-biological interactions 2015 Oct 5;240:336-45
Detection of cresyl phosphate-modified butyrylcholinesterase in human plasma for chemical exposure associated with aerotoxic syndrome.
Schopfer LM, Masson P, Lamourette P, Simon S, Lockridge O
Analytical biochemistry 2014 Sep 15;461:17-26
Analytical biochemistry 2014 Sep 15;461:17-26
A high-throughput diagnostic method for measuring human exposure to organophosphorus nerve agents.
Knaack JS, Zhou Y, Abney CW, Jacob JT, Prezioso SM, Hardy K, Lemire SW, Thomas J, Johnson RC
Analytical chemistry 2012 Nov 6;84(21):9470-7
Analytical chemistry 2012 Nov 6;84(21):9470-7
Immunomagnetic separation and quantification of butyrylcholinesterase nerve agent adducts in human serum.
Sporty JL, Lemire SW, Jakubowski EM, Renner JA, Evans RA, Williams RF, Schmidt JG, van der Schans MJ, Noort D, Johnson RC
Analytical chemistry 2010 Aug 1;82(15):6593-600
Analytical chemistry 2010 Aug 1;82(15):6593-600
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA of Butyrylcholinesterase using HAH 002-01 as the capture antibody and HAH 002-01B as the biotinylated detection antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL